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The present study was carried out with the aim to evaluate the bioactive constituent in Rosa damascena, which is commonly called Demask rose, medicinal plant with biological significance and belongs to Rosaceae. The analyses were performed in methanolic extract with two fraction, Butanol and ethyl acetate. The analysis reveals presence of 09 bioactive compounds. Out of nine compounds, five were derivatives of methyl ester. Chemical compounds found in selected plants possess anti-oxidant potential which indicate potential against cancer disease. Plant also contains biological potential against microbes such as bacteria. Methanolic extract of plant in butanol and ethyl acetate fraction are found to possess significant potential to kill different bacterial species such as Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, and Proteus mirabillis. All these bacterial species were isolated from different sources such as pus, urine, vaginal sweat and sputum. Extract of Rosa damascena was able to kill all above mentioned species but was not capable to destroy Klebsiella oxytoca isolated from urine.

GC-MS analysis of antibacterial compounds from floral part of methanolic extract of Rosa damascene