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RNA is a single stranded nucleic acid type, produced by DNA via a process of transcription. RNA tend to form secondary structure by pairing the complementary base pairs within it. The RNA secondary structure is an essential parameter to understand its functions and expression into a protein. This research is based to understand the link between RNA secondary structure and abiotic stresses in two leading organisms viz; Arabidopsis thaliana and Oryza sativa. For this purpose the RNA secondary structures analyses for abiotic stress resistant and housekeeping genes were done in A. thaliana and O. sativa. Total 138 abiotic stress resistant and 2l housekeeping genes were selected through literature survey and subjected to RNA secondary structure tool. RNA secondary structure were analyzed and characterized in terms of minimum free energy, number of stems and loops. The 80% of abiotic stress and 67% of housekeeping genes show significant difference in the minimum free energy (MFE) while there were no significant variation in the number of stems and loops. The open reading frame analysis, as an additional parameter, of the selected genes were also done, that revealed the preference of +1 frame by most of the genes, i.e., 42% of abiotic stress and 43% of housekeeping genes. RNA secondary structure analysis help us to engineer stress resistant

RNA secondary structure analysis for abiotic stress resistant and housekeeping genes in Arabidopsis aliana and Oryza sativa