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Xylanase have many applications and fruit juice clarification is one of them. The work was done by taking Pseudomonas sp. Xylanase was immobilized on silica with binding efficiency of 46.5% and maximum binding time 6 hours. Maximum amount of juice clarification was in case of ausambi juice was observed at C and 52 hours in both immobilized and free enzyme treated juice, in case of free enzyme treated juice there is 46.9% increase in clarity and 1.7 fold increase in reducing sugars of the juice and enzyme dose was optimized as 8U with maximum flow rate of 6 ml/min at this dose but in case of immobilized enzyme treated juice there is 47.6% increase in clarity and 1.8 fold increase in reducing sugars, ezyme dose optimized is 7.4U with maximum flow rate of 7.23 ml/min at this dose.In case of orange juice in free enzyme treated juice maximum clarity was observed at and hours juice was found to be 42.14 % clear with increase of 1.9 fold of reducing sugars, enzyme dose optimized was 8.06U with maximum flow rate of 0.86 ml/min. In case of immobilized enzyme treated juice maximum clarity was found at 52 hours with 36.46% clarity and 2 fold increase in reducing sugars, ezyme dose optimized was 14.8U with maximum flow rate of 0.9 ml/min at this dose.

Optimization of process parameters for fruit juice clarification using silica immobilized xylanase from Pseudomonas sp.