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Freeze-Dried Powder of Bamboo Juice (FDPBJ) has high edible and medicinal values for abundant polyphenols, terpenoids and other biologically active ingredients. They can lower cholesterol level, regulate fat influent metabolism, prevent and cure Cardiovascular Diseases (CVDs) and so on. The aims of study are to research the lipid metabolism effects of FDPBJ and to develop a new type of raw material for the treatment of fat metabolism diseases. In this research, an accurate, sensitive and rapid in vitro model, steatosis hepatic L02 cell, was applied to assay the activities of FDPBJ on lipid metabolism. The steatosis L02 cells were obtained after being cultured with 1% fat emulsion-10% fetal bovine serum (FBS)-RPMI 1640 medium for 48 h. Contents of triglyceride (TG), Total Cholesterol (TC) and Free Fatty Acid (FFA) in L02 cells were determined after exposure. The results showed that the intracellular TG content was increased from 33.83±2.32 mmol/L to 40.67±2.73 mmol/L in steatosis L02 cells, while the intracellular TC content was increased from 3.48±0.57 mmol/L to 8.83±0.72 mmol/L. At the same time, the exposure of FDPBJ could reduce the TG, TC and FFA contents in steatosis L02 cells significantly. FDPBJ displayed a distinct TG regulation activity and TC regulation activity. In terms of morphology, FDPBJ can clearly reduce the accumulation of lipid droplets in the steatosis liver cell line L02. On account of these in vitro results, FDPBJ might have satisfactory effects in clinic treatment of fat metabolism disease characterized by the elevation of TG and TC.

Lipid Metabolism Effects of Freeze-dried Powder of Bamboo Juice on Liver Cell Line L02 with Steatosis