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Trifloxystrobin, one of the strobilurin fungicides, is active as inhibitor of low toxicity and easy degradation against a wide range of fungal plant pathogens. In the experiment, Hydroxy from magnetic microsphere and amino of anti-Trifloxystrobin antibody could condensate to form Immune Magnetic Beads (IMB), which could specially capture trifloxystrobin in the food matrix and rapidly separated it through the magnetic field. An ELISA method (IMB-ELISA) was developed to detect the captured fungicide through examining the best reactive concentration, sensitivity, specificity, matrix effect and recovery rate. The half maximal inhibitory concentration (IC50) value obtained by the IMB-ELISA method was 2.28μg/mL (R2=0.9837) against 12.57μg/mL of conventional ELISA method (R2=0.9747). The recovery rates were 90.12-103.65% and the Coefficients of Variation (CVs) were in the range of 4.5-11.2%. In three kind of citrus samples, there was only low cross-reactivity in IMB-ELISA method by detecting matrix effects. Based on above experiment, the IMB-ELISA method was an efficient and sensitive method for Trifloxystrobin detection.

Development of an ELISA Method Detecting Strobilurin Fungicide through the Immune Magnetic Beads Collecting Samples