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The kinetic profiles of Oxytetracycline (OTC) in hepatopancreas of Eriocheir sinensis was examined in this research. The results showed that after a single-dose of 40 mg/kg body weight OTC at (18±1°C) by intramuscular administration, the hepatopancreas concentration-time curve of OTC could be best described by a one-compartment model with first-order absorption and a time lag: C = 69.45×(e-0.005(t-0.0387)-e-8.195 (t- 0.0387)). The elimination half-life (t1/2β) was 130.71 h. It is suggested that a withdrawal time of 79.51d should be required before crabs are consumed safely.