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On the basis of single-factor test, we conducted optimization study on the three major factors (material moisture content, cultivation time, temperature) that affect the production of purebred Monascus spores through orthogonal trial. The results show that, in the trial-level range, moisture content of materials and cultivation time have larger effect on the test results, while the cultivation temperature has little effect. The optimum conditions in preparation of purebred Monascus seed koji are: 60% moisture content of materials, 5 d cultivation time, 35°C cultivation temperature, 0.1 mL inoculation, 10 g filling and 0.2% lactic acid. To verify this test scheme, ultimately the number of spores produced by Monascus is 3.35×10^7/g.

Optimization of Process Conditions of Monascus Seed Koji by Orthogonal Test Design