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Lactogenic effect of ethyl acetate fraction of Hibiscus sabdariffa seed was evaluated on serum prolactin and milk production in lactating albino rats. Twenty four lactating rats were grouped randomly at parturition into control, metoclopramide-treated and Ethyl-acetate-treated group consist of six rats in each group (n = 6). The lactating rats were administered control (normal saline), metoclopramide (5 mg/kg) and ethyl-acetate fraction (100 and 200 mg/kg) respectively from day 3-17 of lactation. Milk yield for rats were estimated by pup weight and weight gain. The animals were then euthanized on the day 18 and serum prolactin was analyzed using prolactin kit. The prolactin level of ethyl-acetate fraction of Hibiscus sabdariffa showed a significant increase (p<0.01) when compared to control group. Pup weight gain was also significantly higher (p<0.05) than the control group. This can be deduced that ethyl-acetate fraction of Hibiscus sabdariffa L. seed has lactogenic activity because it increases serum prolactin level and milk production in lactating female albino rats. Hibiscus sabdariffa L. seed stimulate prolactin synthesis; release and milk production in albino rats and it is affordable and safe for consumption.

advance journal of food science and technology/advance journal of food science and technology

Lactogenic Study of the Effect of Ethyl-acetate Fraction of &lt;em&gt;Hibiscus sabdariffa&lt;/em&gt; Linn (&lt;em&gt;Malvaceae&lt;/em&gt;) Seed on Serum Prolactin Level in Lactating Albino Rats