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A new spectroscopic analysis method was built for the determination of green food DNA based on the interaction of o-Hydroxyphenylfluorone (o-HPF) with DNA using the resonance Rayleigh Light Scattering (RLS) technique. In the Tris-HCl buffer at pH 8.1, the RLS signal of o-HPF was enhanced remarkably in the presence of DNA and the enhanced RLS intensity at 519 nm was in direct proportion to DNA concentration in the range of 0.021.8×10 -5 g/L with a good linear relationship. The detection limit was 4.6×10 -8 g/L. The method was simple, rapid and high accuracy which had been applied to the determination of green food DNA in sample with satisfactory results.

Resonance Rayleigh Scattering Method for the Determination of Green Food DNA Based on the Interaction of O-hydroxyphenylfluorone with DNA