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To reveal the relationship between theγ-Glutamyltranspeptidase (GGT) and endogenous formaldehydein shiitake mushroom based on gene level, we cloned the GGT gene from shiitake mushroom. The full-length sequence is 2063 bp contained a 5’-terminal Untranslated Region (UTR) of 36 bp, a 3’-terminal UTR of 113 bp and an ORF of 1914 bp encoding a polypeptide of 637 amino acids, which is high conserved in fungus. Quantitative real-time RT-PCR revealed the change of GGT expression along the growing process of shiitake mushroom, which increased first and then decreased (p<0.05). Simultaneously, the GGT activity and endogenous formaldehyde were determined and had the same trend with mRNA expression (p<0.05). In addition, at each growth stage the GGT expression, activity and formaldehyde were higher in stalks than in caps (p<0.05). The results confirmed the positive relationship between GGT and endogenous formaldehyde production, which hinted that endogenous formaldehyde could be controlled by regulating the expression of GGT and activity.

Gene Cloning of &gamma-Glutamyltranspeptidase and Its Relationship to Endogenous Formaldehyde in Shiitake Mushroom (Lentinus edodes)