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Optimization of conditions for Jujube pectin methylesterase extraction was investigated using orthogonal design methodology. Extraction parameters which are employed in this study are Liquid-Solid Ratio (LSR) (1:5-20), NaCl content (0-2.0 N), ultrasonic time (0-80 min) and they were optimized using a three-factor orthogonal Design coupled with range and variance analysis. The results showed that, NaCl content and solid-liquid ratio have significant effect on the activity of pectin methylesterase. The satisfactory conditions for Jujube pectin methylesterase extraction were obtained as follows: 1:10 of LSR, 1 N of NaCl content and 40 min of ultrasonic time. Among the studied factors, NaCl content had the greatest influence on yield. Under these conditions, the experimental activity of Jujube pectin methylesterase reach the highest, was 0.6841 U/g.

Optimization for Ultrasound-microwave Assisted Extraction of Pectin Methylesterase from Jujube Using with Orthogonal Design Methodology