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In the cerebral and other arteries of dogs of diffcrent ages, histochemical studies have been made on hyaluronic acid and related muccsaccharides by means of digestion procedures with Streptomyc~s and testicular hyaluronidases. In the cerebra! arteries. hyaluronic acid is either scanty in amount or absent, but other arteries contain histochemically demonstrable amounts of this mucosaccharide which decreases, however, with advancing age of the animals. The suppressive effects of digestion with testicular hyaluronidase upon the alcianophilia (pH ],0) of all the al'teries examined indicate that varying amounts of isomericchondroitin sulfates are present in the arterial tissues, The technique of digestion with Streptomyces hyaluronidase established previously 1) has been found to be useful for the histochemical identification of hyaluronic acid in the tissues of dog arteries.

The histochemistry of hyaluronic acid and related mucosaccharides in the cerebral and other arteries of the dog.