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Copyright: © 2006 David Fay. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. To whom correspondence should be addressed. E-mail: davidfay@uwyo.edu Genetic mapping and manipulation: Chapter 9-Synthetic and enhancer mutations* David Fay, Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-3944 USA