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Sejarah arikel: Submisi Revisi Diterima Tersedia online: 4 Oktober 2016 Tea leaves is one of natural product that used as antibacterial. Antibacterial compound can be used as natural preservative. The aim was to determine effectiveness of tea leaves as natural antibacterial to inhibit Pseudomans fluorescens. Tea leaves was extracted by maceration. Separation the extract (catechin) conducted by analytical TLC (chromatography thin layer) using various eluent such as ethyl acetate : aquadest : formic acid (18:1:1), toluene : acetone : formic acid (3:3:1) and chloroform : methanol : aquadest (6.5:3.5:1). Analytical TLC analysis showed the best eluent, ethyl acetate : aquadest : formic acid. The best eluent was used for preparation using preparative TLC. Antibacterial test showed that isolate no.2 was given the highest effectiveness to bacterium P. fluorescens with inhibition zone 23.3 mm.

Efektivitas antibakteri senyawa katekin dari ekstrak daun teh (Camelia sinensis L. var assamica) terhadap bakteri Pseudomonas fluorescens