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A study was conducted to investigate the effect of microencapsulated probiotic consortium containing Lactobacillus plantarum, Enterococcus faecium, Enterococcus hirae, Pediococcus acidilactici and Weissella paramesenteroides on immune modulation in Newcastle Disease vaccinated chicken. Humoral immune response was assessed by ELISA. Th1, Th2 cytokine response and cell mediated immune response were assessed by using Real time PCR and flow cytometry respectively. Results indicated significantly (p less than 0.01) higher antibody titer and also higher IL-2, IL-12, IL-4 and IL-10 cytokine expression in NDV vaccinated multispecies probiotic fed group compared to commercial probiotic fed and control groups. It was also observed that higher proportions of Bu1A (B cell receptor) and CD3 (T cell receptor) positive cells in chicken fed with multispecies probiotic supplementation. Hence, it is concluded that multispecies probiotic played an important role in augmenting humoral and cell mediated immune response against NDV.

Immunomodulatory Potential of Microencapsulated Multispecies Probiotic Consortium in Newcastle Disease Virus Vaccinated Chicken