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Background: The intestinal mucosal epithelium acts as a physical and biochemical barrier and plays an important role in regulating of barrier function and immune homeostasis. Methylmethionine sulfonium chloride (MMSC) is a multifaceted amino acid that is critical to the normal physiology of the gastrointestinal tract. The present study investigated the effects of extracellular MMSC on intestinal epithelial cell line (IPEC-J2).Methods: IPEC-J2 cells were treated with 0.1, 0.5 and 1 mM MMSC, respectively for an additional 24 h. CCK-8 assay was used to evaluate cell proliferation. The cell Annexin V-FITC/PI apoptosis were analyzed by flow cytometry (FCM) method. The mRNA transcript and protein expression levels of tight junction proteins in IPEC-J2 cells were detected by real-time quantitative polymerase chain reaction (RT-qPCR) and western blotting (WB).Result: The results showed that MMSC could stimulate IPEC-J2 cells proliferation and inhibit cell apoptosis. In addition, the RT-qPCR and WB results indicated that 0.5 mM MMSC significantly increased the mRNA and protein expression of tight junction proteins, including occludin, claudin-1 and zonula occludin-1 (Zo-1). These findings may provide valuable information to investigate further the possible mechanism and function of MMCS on the intestinal barrier function.

indian journal of animal research

Effects of Methylmethionine Sulfonium Chloride on Activity and Tight Junction Protein Expression of Intestinal Porcine Jejunum Epithelial Cells (IPEC-J2)