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The present Research trial was conducted at the Research Farm of Division of Horticulture Faculty of Agriculture, Wadura, during Kharif season of 2017 and 2018 to study the effect of Plant Growth regulators (Gibberllic Acid & Cycocel ) on flowering, yield and quality of bitter gourd. Exogeneous application of both GA3 & Cycocel significantly increased the growth and yield of Bitter Gourd with an improvement in quality charecteristics of the fruit over the control. GA3 proved better than the Cycocel in improving the growth Yield and quality characteristics of the Bitter gourd. Application of GA3 60ppm (T3) recorded the Maximum number of branches, Length of vine (cm),Number of fruits per plant , Yield (q/ha) and quality parameters like . Moisture content of fruit (%), TSS (% )and Ascorbic acid (mg/100g pulp).