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To analyse the genetic diversity and relationships among kenaf germplasm, two cultivars and twenty three accessions from different geographical regions were assessed using RAPD markers. Six RAPD primers generated 38 polymorphic bands with an average of 6.33 polymorphic bands per primer. A high level of genetic variation (proportion of polymorphic loci 79.17%) and high genetic distance (0.0426 to 0.6523) were found among 25 indigenous and exotic kenaf germplasm. The UPGMA dendrogram based on Nei’s genetic distance segregated the genotypes into two major, two minor and one single genotype clusters. The distribution pattern of genotypes from different geographical regions into five clusters was random, indicating that geographical isolation may not be only factors causing genetic diversity. The minor cluster III comprised two accessions collected from same origin but they are distantly related to each other. The genetic distance between two cultivars was lower (0.0870) and they were found in the same cluster. These results indicate that studied kenaf germplasm not only exhibit a high level of genetic diversity but also have a different genetic background.

RAPD Analysis of Genetic Diversity and Relationships among Kenaf (Hibiscus cannabinus L.) Germplasm