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The selection of native rhizobia nitrogen (N)-fixing is important for the production of leucaena used in livestock production. In the state of Rio Grande do Sul (RS), Brazil there is little information of native isolates of rhizobia with symbiotic capacity with leucaena plants. The objective of this research was to select native rhizobia, efficient in the biological N fixation in leucaena plants in RS state. Soil and nodule samples were taken at different locations in RS, then leucaena seeds were used to obtain nodules that were processed in culture media to obtain the isolates. An experiment under greenhouse conditions was conducted to evaluate the efficiency of rhizobia isolates. The dry shoot dry mass (SDM), dry mass of the root, number of nodules and the N accumulated N of the plants were determined. The isolates of rhizobia Leu01 and Leu02 were selected as efficient in N fixation because they provide increase in MSPA and an accumulation of N in leucaena plants similar to the control treatment that received N mineral. These isolates could be promising in future greenhouse and field studies of this legume. RESUMO A seleção de rizóbios autóctones fixadores de nitrogênio (N) é importante para a produção de leucena utilizada para a produção pecuária. No estado do Rio Grande do Sul (RS), Brasil, existem poucas informações sobre isolados de rizóbios autóctones com capacidade simbiótica com plantas de leucena. O objetivo deste trabalho foi selecionar isolados de rizóbios autóctones, eficientes na fixação biológica de nitrogênio em plantas de leucena no estado do RS. Foram coletadas amostras de solo e nódulos em diferentes locais do RS e, logo foram utilizadas sementes de leucena para obter os nódulos que em seguida foram processados em meios de cultura para obtenção dos isolados. Foi conduzido um experimento em casa de vegetação para avaliar a eficiência dos isolados de rizóbios, determinando-se a massa seca da parte PALABRAS CLAVE: Simbiosis; Leguminosas; Promoción de crecimiento vegetal.

Rizóbios nativos eficientes en la fijación de nitrógeno en Leucaena leucocephala en Rio Grande do Sul, Brasil