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Gastrointestinal stromal tumors (GISTs) are characterized by oncogenic KIT mutations that cluster in two exon 11 hotspots. The aim of this study was to develop a single, sensitive, quantitative digital droplet PCR (ddPCR) assay for the detection of common exon 11 mutations in both GIST tumor tissue and in circulating tumor DNA (ctDNA) isolated from GIST patients' plasma.

oncotarget

A single digital droplet PCR assay to detect multiple<i>KIT</i>exon 11 mutations in tumor and plasma from patients with gastrointestinal stromal tumors

A single digital droplet PCR assay to detect multipleKITexon 11 mutations in tumor and plasma from patients with gastrointestinal stromal tumors