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Primer sequences were as follows: 5′-ACAGCCACTTTCCACA-3′ sense and 5′-CTGGTGT TAGGGTTCTT-3′ antisense for D-loop, 5′-CCTACT TACAGGGCTCAA-3′ sense and 5′-GAGGGATAGG AGGAGAAT-3′ antisense for Nd4, 5′-CCTCAAGATCA TCAGCAATGC-3′ sense and 5′-TGGTCATGAGTC CTTCCACG-3′ antisense for nuclear DNA encoded glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Annealing temperatures presented above were used as follows: 52°C for D-loop, 49 °C for Nd4, 58°C for Gapdh.

Correction: MtDNA depleted PC3 cells exhibit Warburg effect and cancer stem cell features