LncRNA EPIC1 downregulation mediates hydrogen peroxide-induced neuronal cell injury | Zendy

Jian Sun | Zendy; Jinyu Zheng | Zendy; Ya Li | Zendy; Ming Yan | Zendy; Ping Li | Zendy; Lili Ma | Zendy

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LncRNA EPIC1 downregulation mediates hydrogen peroxide-induced neuronal cell injury

Author(s) -

Jian Sun,

Jinyu Zheng,

Ya Li,

Ming Yan,

Ping Li,

Lili Ma

Publication year - 2019

Publication title -

aging

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.473

H-Index - 90

ISSN - 1945-4589

DOI - 10.18632/aging.102545

Subject(s) - downregulation and upregulation , programmed cell death , ectopic expression , microbiology and biotechnology , apoptosis , cell , chemistry , biology , gene knockdown , cell growth , cell culture , biochemistry , gene , genetics

Excessive oxidative stress causes neuronal cell injury. Long non-coding RNA (LncRNA) EPIC1 (Lnc-EPIC1) is a MYC-interacting LncRNA. Its expression and potential functions in hydrogen peroxide (H 2 O 2 )-stimulated neuronal cells are studied. In SH-SY5Y neuronal cells and primary human neuron cultures, H 2 O 2 downregulated Lnc-EPIC1 and key MYC targets (Cyclin A1 , CDC20 and CDC45 ). Ectopic overexpression of Lnc-EPIC1 increased expression of MYC targets and significantly attenuated H 2 O 2 -induced neuronal cell death and apoptosis. Contrarily, Lnc-EPIC1 siRNA potentiated neuronal cell death by H 2 O 2 . MYC knockout by CRISPR/Cas9 method also facilitated H 2 O 2 -induced SH-SY5Y cell death. Significantly, MYC knockout abolished Lnc-EPIC1-induced actions in H 2 O 2 -stimulated neuronal cells. Together, these results suggest that Lnc-EPIC1 downregulation mediates H 2 O 2 -induced neuronal cell death.

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