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The cytochrome P-450 1A2 (CYP1A2) enzyme accounts for approximately 13% of the CYP family of enzymes. (1) It metabolises drugs and is important for drug-drug interactions. Interindividual di!erences in CYP1A2 phenotype and genotype result in variable enzyme expression and functional activity, thus can alter drug plasma concentration. Ca!eine is largely metabolised by CYP1A2 and is o%en used to investigate an individual’s CYP1A2 metabolic activity, (2) which may help in drug prescription and dose adjustment to avoid toxicity or ine$cacy. (3)

Development of a competitive ELISA and genotype technique to determine caffeine pharmacokinetics and CYP1A2 status in humans