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Cultivation of hybrid groupers (Epinephelus fuscogustatus x Epinephelus lanceolatus), is one of the fish farming activities that have high economic value. Lately the existence of Iridovirus has become a big problem for cultivation. It was a deadly viral disease for grouper hatchery in Asia including Indonesia. Iridovirus may cause mass mortality, especially larvae up to fingerling size. The purpose of this study was to determine the effect of different level of Dunaliella salina against the decrease the number of micronuclei and macronuclei of hybrid grouper. As many as 180 seeds of hybrid grouper with weight 4-5 gram disperse into 15 tanks volume of 16 litres with density of 10 fish/tank. Four treatments were applied with different diets containing powder of Dunaliella salina of K(0 gr kg diet fed), A (2 gr kg diet fed), B (4 gr kg diet fed) dan C (6 gr kg diet fed), with three repetition. Experimental diet was given twice for 10 days. After 10 days of treatment, the fish tested challenge with Iridovirus for 96 hours. The main parameters observed in this study were the number of micronuclei and macronuclei. Based on the research, most of the hematological parameters such as erytrocitess, Hb and HE significantly increased in Dunaliella salina-treated fish compared with controls (P<0.05). Micronuclei and macronuclei after challenge with Iridovirus in 6 mg/kg Dunaliella salina-treated fish was 10.57-14.58 MN/100 cells and 12.02 MAN/100 cells , which significantly decreased compared with control (P<0.05).

russian journal of agricultural and socio-economic sciences

THE EFFECTIVENESS OF DUNALIELLA SALINA AGAINST THE DECREASE MACRONUCLEI AND MICRONUCLEI OF HYBRID GROUPER (EPINEPHELUS FUSCOGUSTATUS X EPINEPHELUS LANCEOLATUS) INFECTED BY IRIDOVIRUS