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It was studied the enzymatic hydrolysis of sugarcane straw in order to obtain fermentable sugars. The biomass passed through physical processes (milling, washing and drying), delignification with H 2 O 2 alkaline and hydrolysis. After characterization of the lignocellulosic biomass in natura and delignified it was observed the removal of 50% of the lignin content. The enzymatic activity of T. reesei cellulase and commercial Cellic® CTec2 was quantified. The reaction pH, enzyme concentration and reaction time were studied, obtaining optimum pH 4.0 and 5.5 for T. reesei and Cellic® CTec2, respectively. The highest yield in terms of enzyme concentration was 10 FPU/g of straw for both enzymes and for the time, both enzymes reduced conversion after 24 hours of hydrolysis, possibly due to microbial contamination. Finally, it has been carried out a material balance for the commercial cellulase, resulting in a yield of the enzymatic hydrolysis of the cellulose content of 23.8%.

ESTUDO DA HIDRÓLISE ENZIMÁTICA DA PALHA DA CANA DE AÇÚCAR PARA PRODUÇÃO DE ETANOL DE SEGUNDA GERAÇÃO