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RESUMEN El nogal (Juglans neotropica) es una especie de gran potencial para proyectos de conservacion y agro-productivos en la eco-region Andina. No obstante, su baja capacidad germinativa limita su propagacion para reforestacion. El objetivo de este estudio fue determinar el efecto del tratamiento con Thidiazuron (TDZ) y 6-Bencilaminopurina (BAP) en la induccion in vitro de brotes de J. neotropica. Las microplantulas tratadas con BAP produjeron mas brotes que aquellas tratadas con TDZ. Las microplantulas tratadas con BAP y TDZ generaron a su vez mas brotes que las no-tratadas. En el ensayo se evaluo tambien el efecto de la concentracion de BAP y TDZ en el medio pero no se detectaron diferencias estadisticamente significativas entre las concentraciones evaluadas. En conjunto, nuestros resultados indican que el tratamiento con cito-quininas, especificamente BAP, incrementa el numero de brotes producidos por microplantula de J. neotropica, pudiendo aplicarse este tratamiento en proyectos de propagacion masiva de esta especie para sostener programas de reforestacion. Palabras clave: Tocte, cedro negro, multiplicacion in vitro. ABSTRACT The walnut (Juglans neotropica) is a species of great potential for conservation projects and eco-agro-production in the Andean region. However, low germination capacity limits its spread for reforestation. The aim of this study was to determine the effect of treatment with Thidiazuron (TDZ) and 6-Benzylaminopurine (BAP) on in vitro shoot induction of J. neotropica. The micro plantlets treated with BAP produced more shoots than those treated with TDZ. The micro plantlets treated with BAP and TDZ in turn generated more shoots than the non-treated micro plantlets. In the test, the effect of concentration of BAP and TDZ in the medium was also evaluated; however not statistically significant differences between the tested concentrations were detected. In summary, findings indicate that the treatment with cytokines, BAP specifically, increases the number of shoots produced by micro plantlets J. neotropica. It is believed that this treatment can be applied on projects of mass propagation of this species within the frame of reforestation programs. Keywords: Tocte, black cedar, in vitro multiplication.

Inducción de la brotación in vitro de microplántulas de Nogal (Juglans neotropica) tratadas con Thidiazuron (TDZ) y 6-Bencilaminnopurina (BAP)