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1 Reader, Department of Conservative Dentistry and Endodontics, Career Post Graduate Institute of Dental Sciences and Hospital, Lucknow 2,3 PG Final Year Student, Department of Conservative Dentistry and Endodontics, Career Post Graduate Institute of Dental Sciences and Hospital, Lucknow 4 Assistant Professor, Department of Microbiology, King George’s Medical University, Lucknow *Corresponding Author Dr Pallavi Mittal PG Final Year Student, Department of Conservative Dentistry and Endodontics, Career Post Graduate Institute of Dental Sciences and Hospital, Lucknow, UP, India Abstract Enterococcus Faecalis is the most common facultative anaerobic bacteria isolated from both secondary and persistent root canal infections. E. faecalis invades and adheres to the dentinal tubules with a depth of penetration ranging from 500 to 1000 μm, and has the ability to survive in harsh environmental conditions due to its potential to transform into the viable but noncultivable (VBNC) state. This Paper highlights the use of Nisin, Calcium Hydroxide and Triple Antibiotic Paste each as an intracanal medicaments combined with propylene glycol, CHX and saline used as a vehicle. Nisin, a chemical commonly used as a food preservative (meat and dairy products), is recently recommended for use as an intracanal medicament. Discovered in 1928, it is a naturally occurring antimicrobial cationic peptide, produced by Streptococcus lactis subspecies lactis. Propylene glycol which was suggested by Laws in 1962 has been evaluated for its possible use as a vehicle in the field of endodontics. It permits the release of calcium and hydroxyl ions essential for the therapeutic action. It has been found to be antibacterial, nonirritating to the periapical tissue. 2% CHX is effective in completely eliminating E. Faecalis from the dentinal tubules up to a period of 15 days attributed to its substantive antimicrobial activity.This study aims to see the combined effect of Nisin, Calcium Hydroxide and Triple Antibiotic Paste when mixed with different vehicle.

In Vitro Evaluation of Antibacterial Efficacy of Nisin Calcium Hydroxide and Triple Antibiotic Paste in Three Different Vehicle