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Department of Microbiology, National Institute of Medical Science and Research (NIMSR), Jaipur *Corresponding Author Bhawani Shankar Verma Department of Microbiology, National Institute of Medical Science and Research (NIMSR), Jaipur Abstract Background: Gram-negative pathogens are an important cause of community and hospital-acquired infections throughout the world. Klebsiella pneumoniae has become one of the more common causes of these infections and one of the important aspects of Klebsiella associated infections is the emergence of multi-drug resistant strains particularly those involved in nosocomial infections or hospital settings. This study was done to determine the isolation rate of Klebsiella, their antibiogram and the presence of resistant strains from sputum samples. Aims and Objective: Isolation and Antimicrobial Susceptibility Pattern of Klebsiella Pneumoniae in a sputum sample with Lower Respiratory Tract infection suspected patients that are study were undertaken to detect the incidence of K. Pneumoniae isolated from different clinical specimens in the Department of Microbiology in NIMS Medical College Jaipur. Materials & Methods: A total number of 270 sputum samples were included in the 6-month study. Klebsiella was identified by standard microbiological techniques and antibiotic susceptibility testing was done by Kirby-Bauer disc diffusion method and interpreted as per CLSI guidelines. Results: A total of 270 cultures from sputum samples and a total of 41 Klebsiella pneumoniae were isolated thus culture positivity was 35.65%, and other organisms were 64.35%. A total of Klebsiella pneumonia isolates, 25 (60.9%) were from males and 16 (39.1%) were from females. Isolation rate was highest in the male patient then female patients were low rate found in this study. The more sensitive to Amikacin (90.24%), Meropenem (85.35%), Imipenem (80.48%). Isolates showed high resistance to Ceftriaxone (73.17%), Levofloxacin (70.73%), and Aztreonam (52.9%) and Ampicillin (100%) because it is intrinsic resistance. Conclusion: The data of this study may be used to determine trends in antimicrobial susceptibilities to formulate local antibiotic policies and overall to assist clinicians in the rational choice of antibiotic therapy.

journal of medical science and clinical research

Isolation and Antimicrobial Susceptibility Pattern of Klebsiella Pneumoniae in sputum sample with Lower Respiratory Tract infection suspected patients at a Tertiary Care Hospital Rajasthan