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1,2 PG & Research Centre in Microbiology, MGR College, Dr MGR Nagar, Hosur, Tamil Nadu India 3,4 PG & Research Centre in Biotechnology, MGR College, Dr MGR Nagar, Hosur, Tamil Nadu India Corresponding Author Dr V. Veeramanikandan Assistant Professor, Research Centre in Microbiology, Dr. M.G.R. Nagar, Hosur635 109, Tamilnadu, India Email: vra.manikandan@gmail.com, Contact: +91-9976307777 Abstract The study of Prevalence and different laboratory diagnosis methods for the determination of typhoid fever in Kashmir region were studied with 676 samples. The samples of patients were classified into three groups, males, females and children from the age group of 8 to 60 years. Out of 676 patients, 250 were males with 146 found positive and 104 were found negative for typhoid. In 350 females samples collected, out of which 239 were found positive for typhoid and 111 has shown negative results. Whereas 76 children sample collected revealed that 28 were found to be positive and 48 were negative for typhoid. The overall results of Widal test showed that out of 413 sample 219 were positive for Antigen O, 165 were positive for Antigen H, 17 were positive for Antigen AH and 12 were positive for Antigen BH. Out of 413 sample, it was found that 146 were both IgM and IgG positive, 35 were both IgM and IgG negative, 192 were IgM positive and 40 were IgG positive. This study revealed that the highest cases were reported with IgM positive whereas as the lowest percentage was found with IgG positive. Out of 192 positive cases (IgM positive for typhidot), 189 were found positive for IgM and 3 were negative by ELISA method. Similarly, out of 40 positive cases (IgG positive for typhidot), 38 were found positive for IgM and 2 were negative by ELISA method. Thus the study found that the prevalence of typhoid was found higher in females than in males and children.

Prevalence and different laboratory diagnosis methods for the determination of typhoid fever in Kashmir region of Jammu and Kashmir, India