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Aim. To investigate specific reactions of induced immunity of callus tissues of tomatoes under conditions of inoculation with bacterial pathogens X. vesicatoria, P. syringae pv. tomato, C. michiganensis subsp. michiganensis. Methods. Histochemical studies of callus structures of tomatoes and specific pathogenesis were carried out on permanent preparations. Results. It was shown that inhomogeneous conditions are created in morphogenic calluses for penetration of bacteria into deep layers of tissue. It was confirmed that the direction and speed of propagation of pathogens in callus depends on the heterogeneity of the structures and the type of tissue formed. It was found that the conduction beams, which in callus are made up of a system of tracheoidal elements and are located in a source of infection, do not contribute to its systemic spread, but are the centers of active resistance to pathogens. Conclusions. A high level of resistance to X. vesicatoria was exhibited by axial structures of the rhizogenic type, which was associated with the functional characteristics of their integumentary tissues. It is determined that bacteria do not overcome the callus-created tissue barriers. In some groups of tomato callus cells, the effect of their resistance against P. syringae pv. tomato was detected in vitro in absence of typical signs of induced immunity, that reveals new aspects in the pathogen-host interaction system.

SPATIAL HETEROGENICITY AND SPECIFICITY OF INDUCED BACTERIOSIS IN CALLUS TISSUES LYCOPERSICON ESCULENTUM MILL. IN VITRO