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Clonal propagation of elite, disease-free coconutpalms is a promising technique for producing uniformplanting material with high yield and diseaseresistance. Over the past few decades cloning of coconuthas been attempted in a number of laboratoriesworldwide; however, success has been limited. In thepresent study, immature inflorescences of 2–12 cmsize were collected from West Coast Tall variety andthe rachilla segments were cultured on four differentmedia combinations in dark condition. Whitetranslucent outgrowths were maximum in Y3 mediumsupplemented with 4.54 μM 2,4-dichlorophenoxyaceticacid (92%) followed by medium 72 with 41.4 μMpicloram, 61.8 μM putrescine and 4.54 μM thidiazuron(TDZ) (87%). After eight weeks in dark, shoot-likeoutgrowth was noticed more in Y3 III (65%) followedby Y3 I. After eight months dark incubation, thecultures were transferred to 1/2 Murashige and Skoog(MS) with two hormone combinations and highfrequency of multiple shoot formation was noticed in1/2 MS with 5.37 μM naphthalene acetic acid (NAA)and 4.44 μM 6-benzylaminopurine (BAP). Maximumshoot development was observed Y3 medium fortifiedwith 5 μM 2-isopentenyl adenine (2ip) and 5 μM BAP.The individual shoots after development of 3–4 leaveswere transferred to 1/2 Y3 medium supplementedwith 5.37 μM NAA and 24.6 μM indole-3-butyric acid(IBA), and root initiation was observed in 39.28%plantlets. Start codon targeted (SCoT) profiling basedon banding pattern of PCR-amplified productsconfirmed the clonal fidelity of in vitro regeneratedcoconut plantlets. The study indicates the possibilityof developing an in vitro regeneration protocol forcoconut using immature inflorescence explants.

<i>In Vitro</i> Regeneration of Coconut Plantlets from Immature Inflorescence