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Presents the results of applying the method of Raman spectroscopy for the qualitative analysis of the surfaces of the heart valve of sheep before and during their decellularization. Optical analysis showed that the implementation of decellularization valves reduces the content of glycosaminoglycans, proteins and lipids. Found that using specified optical coefficients, it is possible to control the efficiency of the process of decellularization heart valves. Keywords: Raman spectroscopy, heart valve, optical coefficient, decellularization, spectral analysis.

Spectral Analysis of Structural Changes of the Heart Valves at Different Stages of Their Decellularization