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In this study, a novel electrochemical sensing principle was developed for adenosine diphosphate (ADP), which utilizes ADP-dependent kinase as the key enzyme. In this principle, ADP-dependent hexokinase, glucose-6-phosphate dehydrogenase, and diaphorase are combined in the presence of glucose, nicotinamide adenine dinucleotide, and hexaammineruthenium (III) as the electron mediator. The reaction was conducted in a homogeneous mixture on a disposable screen-printed carbon electrode. The measurement was completed within 80 s, and ADP was measured between 2 and 50 μM with a detection limit of 1.9 μM (S/N = 3). This simple, rapid method can be generally applicable as an electrochemical assay for the detection of adenosine-triphosphatedependent kinases.

Development of an Electrochemical Adenosine Diphosphate (ADP) Sensing Principle Employing ADP-dependent Kinase