English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

El objetivo principal de esta investigacion fue el desarrollo de una metodologia de propagacion masiva para Vanilla planifolia , asi como la induccion de estructuras vegetales que por sus caracteristicas permitan el desarrollo e implementacion de tecnicas biotecnologicas modernas para el mejoramiento genetico no tradicional de esta especie tropical de gran valor economico y cultural. Como resultado directo de esta investigacion se desarrollo una metodologia de micropropagacion  eficiente y rapida de vainilla a partir de apices radicales. Por otra parte, se lograron establecer las condiciones y protocolos para la formacion de una estructura indiferenciada y totipotente a la cual hemos dado el nombre de callo protocomico, debido a que tiende a formar en ultima instancia brotes y estructuras similares a los PLBs (protocorm-like badies) caracteristicos en diferentes especies de orquideas. Los callos protocormicos se formaron a su vez a partir de una estructura indiferenciada y transitoria generada a partir de los apices radicales cultivados en ausencia de luz, en un medio liquido MS suplementado con 30 g/L de sacarosa, 1 mg/L de BAP y 1 g/L de caseina hidrolizada. Esta estructura a la cual se le dio el nombre de precallo, presento una gran diversidad morfologica en los diferentes tratamientos experimentales utilizados, razon por la cual se establecio una clasificacion para su identificacion. El mayor porcentaje de callos protocormicos se formo en el medio de cultivo base MS solido suplementado con 0.5 mg/L de 2,4-D en oscuridad, con un 72%. Abstract:  The main research objective was the development of an efficient and rapid mass propagation methodology for Vanilla planifolia Jacks. ex Andrews (Orchidaceae), as well as to generate plant structures based on their characteristics of totipotency, undifferentiation and regeneration capacities, which would allow the development and implementation of modern biotechnological techniques for non-traditional genetic improvement of this tropical species, which has an enormous cultural and economic value. As a result of this research, the conditions and protocols for the formation of an undifferentiated and totipotent structure were established, which was named protocormic callus, because it tends to form shoots and similar structures in appearence to those PLBs (protocorm-like bodies) characteristic of different species of orchids, under regeneration conditions. The protocormic callus were formed from a transitory and undifferentiated structure generated by root tips cultivated without the presence of light, in a liquid MS medium supplemented with 30 g/L of sucrose, 1 mg/L BAP and 1 g/L of hydrolyzed casein. This structure was named pre-callus and presented a great morphological diversity when different experimental treatments were used, for this reason a morphotypic classification was established. Most of the protocormic callus formed in a solid MS basal medium with 0.5 mg/L of 2,4-D in the dark, with 72% (p<0.05). Two vanilla mass micropropagation methodologies, through indirect organogenesis (by pre-callus and protocormic callus induction), were established for the rapid and big scale reproduction of this species from a unique explant, which produced a high number of plants and decreased the time for their obtention. High propagation from pre-callus allows continuous autopropagation sustained in an exponential way through several consecutive cycles of multiplying, due to the ability that these structures present to form a big amount of shoots, which also develop roots, which form pre-callus again, in the same  culture cycle, in liquid medium.

polibotánica/polibotánica

PROPAGACIÓN MASIVA Y FORMACIÓN DE CALLOS PROTOCÓRMICOS DE VAINILLA A PARTIR DE ÁPICES RADICALES VANILLA MASS PROPAGATION AND PROTOCORMIC CALLUS FORMATION FROM ROOT TIPS