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The amount of Microsystis cells at the entrance of rivers to southern Lake Taihu has important influence on the drinking water security and water quality to county nearby and Lake Taihu A real⁃time fluorescence quantitative PCR method was estab⁃ lished using the microcystin synthetase gene mcyE / ndaF as target in this study The quantity of Microsystis cells at the entrance of rivers to southern Lake Taihu was assayed Results showed that the specificity, accuracy and repetitiveness of the method were good The standard curve to detect Microcystis aeruginosa was y=-3 454x+49 88 The slope, correlation coefficient and amplica⁃ tion efficiency were -3 454, 0 991 and 94 6% , respectively It was revealed that the amount of microcystin producing blue⁃green algae at site of Jiapu and Hexi was the highest among the 7 sampling sites, reaching (1 99±0 35)×105 and (1 47±0 23)×105 cells / ml, respectively The main microcystin producing species was Microcystis aeruginosa in all the sampling sites This method could be used rapidly and accurately to detect the quantity and type of the microcystin producing blue⁃green algae, and provide evi⁃ dence for algal bloom and early warning.

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Detection of microcystin-producing <i>Microcystis</i> cells at the entrance of rivers to southern Lake Taihu by real-time fluorescence quantitative PCR

Detection of microcystin-producing Microcystis cells at the entrance of rivers to southern Lake Taihu by real-time fluorescence quantitative PCR