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Distribution of soluble reactive phosphate (SRP) in pore waters is a sensitive indicator showing geochemical properties of phosphorus in sediments. Concentration of SRP in synthetic solutions was obtained based on the principle of diffusive equilibration in thin films (DET). Analytical procedure generally includes equilibration of the gels in solutions or pore waters, section of the gels after retrieval, back elution of phosphorus in the gels, and analysis of SRP in the eluents. Equilibrium and extraction times were determined at 24h and 16h, respectively, with 0.25mol/L HNO3 used as an extractant. Concentrations of SRP in pore waters of different sediments measured by using the established DET technique agree well with its actual values, and the variations are generally within ±5%. Analytical results for sediment profiles with this technique, which has a vertical resolution at about 3mm, also show comparison with those using sampling techniques of Rhizon and Mini-Peeper. The established DET technique was used to measure SRP profiles in sediments of macrophyte- and algal-dominated regions in Lake Taihu. Peak-shape distribution and horizontal heterogeneity of SRP were observed in macrophyte-dominated region, while an upward and increasing movement of SRP was observed in algal-dominated region with an increase in water temperature.

Determination of soluble reactive phosphorus in porewaters of sediments using the technique of diffusive equilibration in thin films