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4 Introduction 4 Material and Methods 16 Bacterial Isolates and Materials 16 Biofilm Quantitation 17 Choosing Material for Optimized Biofilm Formation 17 Co-culture Biofilm Formation Assay Optimization17 Sonication and Drip Dilutions18 Standardizing Viable Cell Count 19 Co-culture Biofilm Assays 20 Co-culture biofilms of five CF pathogens in pairwise combinations 20 The effect of B. cenocepacia J2315 on multiple S. aureus strains 20 Pre-established S. aureus biofilm and B. cenocepacia J2315 21 Fractioning B. cenocepacia J2315 cultures 23 Sonication of B. cenocepacia J2315 cells and biofilm assay 24 Phenotypes of Co-culture Biofilms 24 B. cenocepacia J2315 electrocompetent cells 25 Transformation of B. cenocepacia J2315 26 CLSM preparation and analysis 26 Statistical Analysis 27

Burkholderia cenocepacia J2315-mediated destruction of Staphylococcus aureus NRS77 biofilms.