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This work is devoted to the study of human blood protein fractions by Raman spectroscopy. Whole blood and blood plasma were used as the tested samples. For the pure Raman spectra analysis the autofluorescence background was subtracted by using of two mathematical approaches: polynomial approximation and baseline correction with asymmetric least squares. The study allowed for revealing the differences between the spectral features of blood plasma and whole blood plasma, which are changes in the relative Raman intensities of plasma and whole blood and appearance Raman bands 670 cm -1 , 750 cm -1 , 1120 cm -1 and 1550 cm -1 correspond to hemoglobin bonds in whole blood. The spectral features were used for total protein concentration measurement of plasma and whole blood. PLS regression method was utilized for spectral data analysis with different protein concentrations. The VIP-scores make it possible to determine the most informative spectral bands: 1002 cm -1 , 1227 cm -1 , 1400 cm -1 , 1630 cm -1 for proteins analysis.

Raman spectra analysis of human blood protein fractions using the projection on latent structures method