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Bioinformatics is a field of study having an enormous potential, allowing to solve a number of problems arising as a result of dynamic development of natural sciences with the use of computer science methodologies. It is widely used and constitutes a basis for most scientific research conducted in the field of molecular biology. The aim of this study was in silico analysis of the promoter sequence of the 9f-2.8 gene encoding isoform of the germin protein considered as a germination marker in common wheat’s (Triticum aestivum L.). The gene mentioned above has already been characterized, however, with the use of experimental methods instead of bioinformatics. Analysis with the use of TSSP and TSSPlant software identified the promoter region and classified it as the TATA-box containing promoter. For 9f-2.8 gene including 2.8 kbp, the TSSP software indicated that the TATA-box sequence was located in the position 1665 nt, while the TSSPlant tool showed that TSS [+1] was located in the position 1699 nt. At the second stage, transcription factors were analyzed. Four main families of transcription factors were detected within the analyzed region: MADS, AP2, bZIP and NAC. The most common were MADS-box and bZIP motifs. In the final step of analysis the presence of CpG islands have been checked using the PlantPAN software. The region which could be potentially considered as CpG island have been detected and localized. Software used in analysis above is free online tool. Analiza bioinformatyczna sekwencji promotora genu 9f-2.8 kodującego germinę Słowa kluczowe Triticum aestivum L., narzędzia bioinformatyczne, białko germina, analiza in silico Streszczenie Bioinformatyka jest dyscypliną nauki, w której tkwi olbrzymi potencjał. Dyscyplina ta rozwiązuje wiele problemów powstałych w wyniku dynamicznego rozwoju nauk przyrodniczych przy użyciu metodologii nauk informatycznych. Ma szerokie zastosowanie i jest bazą dla prowadzenia większości badań naukowych z dziedziny biologii molekularnej. Celem artykułu jest analiza in silico promotora genu 9f-2.8 kodującego izoformę (9f-2.8) białka germiny uważaną za marker kiełkowania u pszenicy zwyczajnej (Triticum aestivum L.). Gen ten był już wcześniej scharakteryzowany, jednak do jego analizy korzystano z metod eksperymentalnych, nie obliczeniowych. Analiza bioinformatyczna za pomocą programów TSSP i TSSPlant pozwolila zidentyfikować promotor i potwierdziła jego klasyfikacje do grupy promotorów z motywem TATA-box. W genie 9f-2.8 liczącym 2.8 kpz program TSSP wykazał, że sekwencja TATA-box #0#

Bioinformatics analysis of the promoter sequence of the 9f-2.8 gene encoding germin