English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

El objetivo fue determinar la adicion del 5% de dioxido de carbono (CO 2 ) a partir de granulos efervescentes o gasificado a una incubadora portatil (taper) para evaluar la produccion in vitro de embriones de ovocitos aspirados de ovarios de vacas sacrificadas en el matadero y evaluar la viabilidad in vivo a traves de la gestacion en vacas receptoras. Como incubadora portatil se utilizo un taper de plastico de capacidad de 500 cm 3 a la que se acondiciono una valvula para el control de adicion de CO 2 . La maduracion se realizo por 24 h, fertilizacion por 18 h y cultivo de embriones por 7 dias en 500 uL de los medios en placas petry e introducidos dentro la incubadora portatil, la misma que se coloco dentro un bano Maria a 38,5 o C. A las 72 h post fertilizacion se determino la proporcion de cigotos, a los 7 dias post cultivo se contabilizo los embriones. Los resultados de fertilizacion con adicion de CO 2 a partir de granulos efervescentes fue 38,28% (103/269), con adicion de CO 2 gasificado 37,24% (146/392) y control 49,82% (144/289) (p 0,05); la tasa de gestacion en vacas receptoras fue 38,46% (5/13). En conclusion, es posible producir embriones in vitro de vacunos, utilizando una incubadora portatil de CO 2 a nivel de campo. Palabras clave : fertilizacion, transferencia embrionaria, gestacion. ABSTRACT The objective was to determine the addition of 5% carbon dioxide (CO 2 ) from effervescent granules or gasified to a portable incubator (taper) to evaluate the in vitro production of embryos of oocytes aspirated from ovaries of cows slaughtered in the slaughterhouse and evaluate viability in vivo through pregnancy in recipient cows. As a portable incubator, a 500 cm 3 plastic taper was used, to which a valve for controlling the addition of CO 2 was conditioned. The maturation was carried out for 24 h, fertilization for 18 h and embryo culture for 7 d in 500 uL of the media in petry plates and introduced into the portable incubator, which was placed in a water bath at 38.5 ° C. At 72 h post fertilization the proportion of zygotes was determined, at 7 d post culture the embryos were counted. The fertilization results with the addition of CO 2 from effervescent granules was 38.28% (103/269), with the addition of gasified CO 2 37.24% (146/392) and control 49.82% (144/289) ( p 0.05); the pregnancy rate in recipient cows was 38.46% (5/13). In conclusion, it is possible to produce in vitro embryos of cattle, using a portable CO 2 incubator at field level. Keywords : fertilization, embryo transfer, pregnancy.

Producción de embriones in vitro en una incubadora portatil de dióxido de carbono y viabilidad in vivo post transferencia a vacas receptoras