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CD10 is a metalloproteinase (Zinc dependent) which is expressed in stroma of various epithelial malignancies. It is associated with biological aggressiveness of the tumor. Till now very few studies has been undertaken correlating CD10 with other prognostic markers like lymphnode status, tumor grade, stage, ER, PR, HER2-neu and ki67 in breast carcinoma. Aim: To see the CD10 expression in breast carcinoma and correlate it with ER, PR, HER2 neu and ki67. Materials and Methods: 55 cases of carcinoma breast were diagnosed on histology. Tissue microarray blocks were prepared and Immunohistochemistry was performed for ER, PR, HER2neu, Ki67 and CD10. Result: 32(58%) cases are CD10 positive and 23 (42%) cases are CD10 negative. Out of this 32 positive cases, 12 (22%) cases are weak positive and 20 (36%) cases are strong positive. CD10 is positively correlated with tumor grade.CD10 is negatively correlated with ER and PR status which is not statistically significant and no correlation is found between CD10 and HER2-neu. A positive correlation is seen between CD10 and ki67, but it is not statistically significant. Conclusion: CD10 expression is positively correlated with Ki67 and increasing tumor grade and whereas inversely correlated with ER and PR status. No correlation is seen between CD10 with lymph node status and HER2-neu. Introduction Carcinoma breast is a growing menace world wide. It is taking its toll relentlessly. It is estimated that about 1,15,000 new patients are added and approximately 53,000 deaths occure in every year1 due to carcinoma of breast. Human breast carcinomas are a heterogenous group of tumors that are diverse in behavior, outcome and response to therapy. prognostic factors like lymphnode status, grade, stage, ER, PR, HER2 neu are routinely performed in breast carcinoma. Though Breast carcinoma is an malignancy of epithelial origin, but stroma plays an important role in pathogenesis of progression of tumor and metastasis. Proliferation rate is also found to be a good predictor of aggressiveness of the tumor. Hence new markers such as Ki 67 gene over expression and CD10 have been added to the armament for the prognostication of these tumors. More recently, a combination of CD10 with the established four markers (ER, PR, HER2-neu, Ki67) has been shown to have a strong prognostic impact that is similar to that of gene expression assays that has been described in many studies. CD10 is a metalloproteinase(Zinc dependent). It is expressed in mature neutrophils, Pro B lymphoblast and bone marrow lymphoid stem cells. Various studies also revealed that stromal CD10 expression is associated with biological aggressiveness in many epithelial malignancies. Till now very few studies have been done correlating expression of CD10 with ER, PR, HER2neu and only one study has been done correlating CD10 with Ki67.So our aim was to study the stromal CD10 expression in breast carcinoma and correlating CD10 with ER, PR, HER2neu and Ki67. Materials and Methods Study was done in the Department of Pathology, KIMS, Bhubaneshwar, Odisha from September 2015 to August 2017. It is a prospective study. 55 cases of carcinoma breast were selected on which modified radical mastectomy was performed and diagnosed on histology as invasive breast carcinoma, NST. The clinical data like age, gender, laterality of the tumor were noted. Grading and pathological TNM staging was done. Microarray blocks were prepared from the donor tissue blocks as described by Nazar et al. The desired area of tumor tissue was selected manually. Tissue microarray blocks are constructed. cylindrical tissue core biopsies was extracted from different donor paraffin blocks and re-embedded these in to single recipient block. Immunohistochemistry was performed for ER, PR, HER2Article Info Received: 27th November, 2018 Accepted: 4th February, 2019 Published Online: 9th August, 2019

indian journal of pathology and oncology

CD10 expression by stromal cells in carcinoma of breast and its correlation with ER, PR, HER2neu and Ki67-A tissue microarray study in a tertiary care hospital