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Low-density Lipoprotein Cholesterol (LDL-C) concentration in blood gives indication of cardiovascular disease risk. LDL-C may vary at various strata of Triglycerides and in metabolic diseases like Diabetes Mellitus. Measurement of LDL-C directly on laboratory instruments is the best method but is costly. It can also be estimated using various formulae. Our study involves the comparison of LDL-C measured directly with LDL-C estimated by calculations using various formulae. Materials and Methods: Lipid profile data was collected from Central Clinical Laboratory of MIMER Medical College and hospital, Talegaon Dabhade. Serum LDL-C values were estimated by calculation from Triglycerides (TG), Total Cholesterol (TC) and High-density lipoprotein cholesterol (HDL-C) values, using Friedewald’s, Cordova and Cordova, Vujovic, Anandaraja, Puavillai, and Hattori formula. Data of Direct LDL-C and LDL-C estimated by various formulae were compared and correlated at various levels of TG. Result: Puavillai formula correlates the best with Direct LDL-C at TG </= 150 mg/dL. At TG 151-199 mg/dL, Friedewald’s formula, while at TG 200-399 mg/dL, Anandaraja formula is the best. At all these TG levels, Puavillai formula correlates the best with Direct LDL-C. Conclusion: Puavillai formula is the most accurate formula to calculate LDL-C at TG levels up to150 mg/dL and also at all TG levels studied. Friedewald’s formula is the best at TG 151 to 199 mg/dL and Anandaraja formula at TG 200 to 399 mg/dL in our study population.

Comparison of LDL-cholesterol estimated by various formulae with directly measured LDL-cholesterol in a tertiary care centre of Maval Taluka