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In immunity functional tests with flow cytometry, proteins emerging surface or inner part of lymphocytes are analyzed with the aid of fluorescent emission generally. In this study, we employed newly prepared Schiff base binuclear Zn(II) complexes instead of commercially available fluorescent labeling agents to improve intensity of fluorescence. According to UV-vis, fluorescent, and flow cytometry measurements, X-ray crystallography, and in-silico docking simulation (GOLD), it can remarkably enhance fluorescent intensity for CD19 antibody without specific binding to CD19.

Schiff Base Dinuclear Zn(II) Complexes as Assisting Fluorescent Probe for CD19 Antibody Protein