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The regrowth of perennial ryegrass was harvested on various dates in a pot culture experiment for the purpose of studying the nitrogen, protein and nitrate content and the cation-anion ratio in the yield at different stages of regrowth. Nitrate accumulation occurred until a specific yield level was reached which was cri tical with respect to the nitrogen supply. Below this critical yield the nitrogen percentage in the yield gradually declined as a result of the decreasing capacity of the dry matter produced to assimilate nitrogen. After the critical yieldwas exceeded' nitrate disappeared from the leaves and no further nitrogen was taken up during the subsequent growth, so that the nitrogen percentage in the yield decreased more rapidly. At first there was a gradual increase in the cation-anion ratio as the grass aged. After the critical yield for nitrogen depletion was exceeded there was a higher rate of increase. The relationship between cation-anion ratio and nitrogen status is connected with ni trogen metabolism.

Nitrate accumulation, nitrogen balance and cation-anion ratio during the regrowth of perennial ryegrass.