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viii LIST OF TABLES xv LIST OF FIGURES  xvi LIST OF ABBREVIATIONS  xxii CHAPTER ONE: CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS INFECTION AND ITS PREVENTION BY VACCINE 1 Background and Introduction 1 Staphylococcus Aureus “the super bug” 1 Virulence and Pathogenicity of S. aureus 3 S. aureus Vaccine Antigens and Vaccines 10 Current Vaccines Against S. aureus Human Disease 15 Bovine Mastitis 16 Treatments and Prevention of Bovine Mastitis 21 Cholera Toxin as an Adjuvant for Subunit Vaccines 24 Native Ct: Immunostimulatory Effects 26 Non-Toxic CT Derivatives 28 Summary and Objectives 30

Development of a Cholera Toxin CTA2/B Based Staphylococcus aureus Vaccine to Prevent Bovine Mastitis