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In the total fraction of low and very low density lipoproteins (LDL+VLDL) isolated from serum by precipitation in the presence of heparin-Mn the copper-induced lipid peroxidation was accompanied by accumulation of LPO products, a decrease ANS fluorescence intensity (FANS) and an increase in probe - cation DSP-6, a fluorescence intensity decrease of intrinsic in the ultraviolet area (Fuv) and an increase in the visible area (Fvis). The degree of lipoprotein modification was estimated by calculating the Fvis / Fuv and FDSP-6 / FANS ratio. Strong positive correlation was found between these ratios and concentration of thiobarbituric acid-reactive substances (TBARS) of LDL+VLDL samples isolated from sera of 49 donors and incubated at 37°С in the presence of 50 M CuSO4 during 0, 3 and 24 hr (Fvis / Fuv (r=0,75; p&lt;0,001) and FDSP-6 / FANS (r=0,73; p&lt;0,001)). Very strong positive correlation was also found between both fluorescent parameters Fvis / Fuv and FDSP-6 / FANS (r=0,95, p&lt;0,001). Changes in the values of Fvis / Fuv, FDSP-6 / FANS, concentration of TBARS in 75 patients with documented coronary heart disease (CHD) and 49 apparently healthy donors were studied. No significant differences of these parameters in LDL+VLDL of patients with CHD and donors were found.

biomedicinskaâ himiâ

Investigation of peroxide modification of low- and very low-density lipoproteins in patients with coronary heart disease by the method of fluorescent spectroscopy