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This study aimed to prepare an experimental nano vaccine for Acinetobacter baumannii. In current study, Acinetobacter isolates were identified by growth onto CHRO Magar TM Acinetobacter/MDR and standard biochemical tests. Identification of isolates of Acinetobacter isolates to species level was done by VITEK 2 compact system and API20. Chitosan nanoparticles was used in this study as an adjuvant, the results showed chitosan a perfect adjuvant because it carried outer membrane protein antigen and stimulates an immune response at treated rats, use of Chitosan nanoparticles as a carrier to OMP increased the concentration of cytokines (IL-2, IL-6, IFN-ƴ), antibody titer, total leukocytes and differential leukocytes in treated rats compared with each control group and OMP group.

Immunogenic properties of outer membrane protein of Acinetobacter baumannii that loaded on chitosan nanoparticles