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The study was aimed to develop efficient shoot regeneration from ex vitro explants of Carissa opaca , an imperative medicinal reservoir. Shoot apices and nodal segments were inoculated on MS (Murashige and Skoog) medium containing BAP (6-bezyl amino purine) and Kin (Kinetin) alone and in combination with NAA (naphthalene acetic acid) and GA 3 (Gibberellic acid). Higher concentrations of both cytokinins were found effective for regeneration from both explants. However, gibberellic acid and NAA addition with cytokinin, no persuading results were achieved. The shoot apices were found more effective in in vitro regeneration than nodal segments.The protocol can be effectively used for in vitro multiplication of C. opaca , genetic transformation, and secondary metabolite production.

<i>In Vitro</i> Regeneration of Medicinally Important Shrub <i>Carissa Opaca</i> from Shoot Apices and Nodal Segments