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The identification and quantification of phenolic compounds of  Stachys lavandulifolia  Vahl. var.  brachydon  Boiss. by LC-MS/MS (Liquid Chromatography- tandem Mass Spectrometry) technique is the main purpose of the current study. The high concentrations of quinic acid (2534±12 ppb) and chlorogenic acid (1882±92 ppb) were detected by LC-MS/MS.. Another goal of the study is to evaluate the antioxidant activities of both ethanol and aqueous extracts of the plant material. The antioxidant potentials of extracts were determined by using five different  in vitro  methods including; ABTS (2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid), DPPH(1,1-diphenyl-2-picryl-hydrazyl), FRAP (Ferric ions Reducing Antioxidant Power), CUPRAC (Cupric ions Reducing Antioxidant Power), and ferric thiocyanate methods. The results revealed that the aqueous and ethanol extracts of  S. lavandulifolia  leaves have good antioxidant potential with high phenolic content.

international letters of natural sciences

LC-MS/MS Analysis of Phenolic Compounds and &lt;i&gt;In Vitro&lt;/i&gt; Antioxidant Potential of &lt;i&gt;Stachys lavandulifolia &lt;/i&gt;Vahl. var.&lt;i&gt; brachydon &lt;/i&gt;Boiss.