English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

O objetivo deste trabalho foi determinar um protocolo de micropropagacao da especie jequitiba-branco ( Cariniana estrellensis ) . As sementes foram incubadas em meio Murashige e Skoog (MS). O delineamento utilizado foi o inteiramente casualizado. Os tratamentos de desinfestacao consistiram de imersao das sementes em solucao de hipoclorito de sodio (NaClO) por diferentes tempos (5, 10, 15 e 20 minutos). Para a multiplicacao in vitro, apices caulinares, obtidos de vitroplantas, foram inoculados em meio de cultura MS contendo diferentes concentracoes (0; 0,5; 1,0; 2,0 mg L -1 ) de 6-benzilaminopurina (BAP) e cinetina (KIN). No enraizamento in vitro das brotacoes foram utilizadas diferentes concentracoes de acido indol-3-butirico (AIB) e acido 1-naftaleno acetico (ANA) (0; 0,5; 1 mg L -1 ). A incubacao dos tubos foi realizada no claro em sala de crescimento. Observou-se que a desinfestacao utilizando NaClO por um periodo de tempo de 5 minutos proporcionou melhor descontaminacao e germinacao das sementes. A utilizacao de 1 mg L -1 de BAP adicionada ao meio de cultivo foi eficaz para a formacao de brotacoes na etapa de multiplicacao in vitro. A adicao de 0,5 mg L -1 da auxina AIB ao meio de cultivo proporcionou melhor formacao de raizes nas brotacoes, que apos 30 dias de aclimatizacao, tiveram uma taxa media de sobrevivencia de 65%. Portanto e necessaria a utilizacao de reguladores de crescimento para a multiplicacao in vitro de C. estrellensis , sendo possivel obter mudas micropropagadas da especie nas condicoes do presente trabalho.  Palavras-chave: florestais nativas; germinacao; micropropagacao. IN VITRO PROPAGATION OF “JEQUITIBA-BRANCO” ( Cariniana estrellensis ): AN ALTERNATIVE FOR REFORESTATION PROGRAMS ABSTRACT The objective of the present work was to establish a micropropagation protocol for “jequitiba branco” ( Cariniana estrellensis ). Seed explants were plated on Murashige and Skoog medium (MS). The design was completely randomized. Disinfection treatments consisted of seed immersion in sodium hypochlorite solution (NaClO) for different time periods (5, 10, 15 and 20 minutes). For in vitro multiplication, apical segments obtained from vitro-plants were inoculated into MS culture medium containing different concentrations (0; 0.5; 1.0; 2.0 mg L -1 ) of 6-benzylaminopurine (BAP) and Kinetin (KIN). In vitro rooting of shoots were used different indole-3-butyric acid (IBA) and 1-naftalen acetic acid (NAA) (0; 0.5; 1 mg L -1 ) concentrations. Incubation of the tubes was carried out inside the growth room with artificial lighting. It was observed that disinfestation using NaClO for 5 minutes provided better decontamination and seed germination. One milligram per liter of BAP added to the culture medium was effective for the shoot formation in the in vitro multiplication. The addition of 0.5 mg L -1 of auxin IBA to the culture medium provided better root formation in shoots, which had an average survival rate of 65% after 30 days of acclimatization. Therefore, it is necessary to use growth regulators for the in vitro multiplication of C. estrellensis , making it possible to obtain micropropagated seedlings of the specie in this work conditions.

PROPAGAÇÃO IN VITRO DE JEQUITIBÁ-BRANCO (Cariniana estrellensis): UMA ALTERNATIVA PARA PROGRAMAS DE REFLORESTAMENTO